                         SEQUENCE LISTING

<110>  Nature Technology Corporation
 
<120>  REPLICATIVE MINICIRCLE VECTORS WITH IMPROVED EXPRESSION

<130>  RMVWIE111912

<150>  US61796765
<151>  2012-11-19

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: HTLV- IR-Rabbit Beta globin 
       hybrid intron

<400>  1
aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac       60

ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tagttctctc      120

gttaacttaa tgagacagat agaaactggt cttgtagaaa cagagtagtc gcctgctttt      180

ctgccaggtg ctgacttctc tcccctgggc ttttttcttt ttctcagg                   228


<210>  2
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: HTLV- IR CMV hybrid intron

<400>  2
aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac       60

ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tagttaacgg      120

tggagggcag tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata      180

gctgacagac taacagactg ttcctttcca tgggtctttt ctgcagt                    227


<210>  3
<211>  829
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CMV intron

<400>  3
acgtaagtac cgcctataga ctctataggc acaccccttt ggctcttatg catgctatac       60

tgtttttggc ttggggccta tacacccccg cttccttatg ctataggtga tggtatagct      120

tagcctatag gtgtgggtta ttgaccatta ttgaccactc ccctattggt gacgatactt      180

tccattacta atccataaca tggctctttg ccacaactat ctctattggc tatatgccaa      240

tactctgtcc ttcagagact gacacggact ctgtattttt acaggatggg gtcccattta      300

ttatttacaa attcacatat acaacaacgc cgtcccccgt gcccgcagtt tttattaaac      360

atagcgtggg atctccacgc gaatctcggg tacgtgttcc ggacatgggc tcttctccgg      420

tagcggcgga gcttccacat ccgagccctg gtcccatgcc tccagcggct catggtcgct      480

cggcagctcc ttgctcctaa cagtggaggc cagacttagg cacagcacaa tgcccaccac      540

caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg tctgaaaatg agcgtggaga      600

ttgggctcgc acggctgacg cagatggaag acttaaggca gcggcagaag aagatgcagg      660

cagctgagtt gttgtattct gataagagtc agaggtaact cccgttgcgg tgctgttaac      720

ggtggagggc agtgtagtct gagcagtact cgttgctgcc gcgcgcgcca ccagacataa      780

tagctgacag actaacagac tgttcctttc catgggtctt ttctgcagt                  829


<210>  4
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free intron I 140

<400>  4
ggtaagtcac tgactgtcta tgcctgggaa agggtgggca ggagatgggg cagtgcagga       60

aaagtggcac tatgaaccct gcagccctag gaatgcatct agacaattgt actaaccttc      120

ttctctttcc tctcctgaca gg                                               142


<210>  5
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Human Beta globin Murine IgG 
       chimeric intron

<400>  5
ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag       60

acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc      120

ctttctctcc acagg                                                       135


<210>  6
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Adenovirus leader- Murine IgG
       chimeric intron

<400>  6
gattcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg       60

acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc      120

gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg      180

tcaagcttga ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc      240

actttgcctt tctctccaca ggtgtccact cccagg                                276


<210>  7
<211>  128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Rabbit Beta globin intron

<400>  7
ggtaagtatc ctttttacag cacaacttaa tgagacagat agaaactggt cttgtagaaa       60

cagagtagtc gcctgctttt ctgccaggtg ctgacttctc tcccctgggc ttttttcatt      120

ttctcagg                                                               128


<210>  8
<211>  273
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Truncated CMV intron

<400>  8
acgtaagtac cgcctataga ctctataggc acaccccttt ggctcttatg catgctatac       60

tgtttttggc ttggggccta tacacccccg cttccttatg ctataggtga tggtatagct      120

tagcctatag gtgtgggtta ttgaccatta ttgaccactc caacggtgga gggcagtgta      180

gtctgagcag tactcgttgc tgccgcgcgc gccaccagac ataatagctg acagactaac      240

agactgttcc tttccatggg tcttttctgc agt                                   273


<210>  9
<211>  921
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CAG (Chicken Beta 
       Actin-rabbit beta globin) intron

<400>  9
ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg       60

gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg      120

cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg      180

gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg      240

cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg      300

ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg      360

cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca cggcccggct      420

tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg      480

cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag      540

gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc      600

ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tggcggagcc      660

gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg gtgcggcgcc      720

ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc      780

catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga cggggcaggg      840

cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg      900

ccttcttctt tttcctacag c                                                921


<210>  10
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CMV-Rabbit Beta globin hybrid
       intron

<400>  10
aggtaagtag ttaacttaat gagacagata gaaactggtc ttgtagaaac agagtagtcg       60

cctgcttttc tgccaggtgc tgacttctct cccctgggct tttttctttt tctcagg         117


<210>  11
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K gamma Origin

<400>  11
ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt       60

ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt      120

gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc      180

ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat      240

gagagcttag tacgtactat caacaggttg aactgctgat c                          281


<210>  12
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free R6K gamma origin

<400>  12
aaaccttaaa acctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct       60

tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt      120

acatgaaaca tgagagctta gtacattagc catgagagct tagtacatta gccatgaggg      180

tttagttcat taaacatgag agcttagtac attaaacatg agagcttagt acatactatc      240

aacaggttga actgctgatc                                                  260


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: ColE2 Origin (+7)

<400>  13
caaaagggcg ctgttatctg ataaggctta tctggtctca ttttg                       45


<210>  14
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: ColE2 Origin (Min)

<400>  14
ggcgctgtta tctgataagg cttatctggt ctcatttt                               38


<210>  15
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: ColE2 origin (Core)

<400>  15
ggcgctgtta tctgataagg cttatctggt ct                                     32


<210>  16
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free ColE2 Origin (+7, 
       CpG free)

<400>  16
caaaaggggg ctgttatctg ataaggctta tctggtctca ttttg                       45


<210>  17
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free ssiA [from plasmid 
       R6K]

<400>  17
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aaccca                                                       76


<210>  18
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: +7(CpG free) ColE2 origin-CpG
       free ssiA

<400>  18
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggggctgt tatctgataa ggcttatctg gtctcatttt      120

g                                                                      121


<210>  19
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: +7(CpG free) ColE2 origin-CpG
       free ssiA -flanked by SphI and KpnI sites

<400>  19
gcatgctaca atggctcatg tggaaaaacc attggcagaa aaacacctgc caacagtttt       60

accacaattg ccacttaacc cacaaaaggg ggctgttatc tgataaggct tatctggtct      120

cattttggta cc                                                          132


<210>  20
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA-OUT Selectable Marker

<400>  20
gtagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta       60

ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg      120

attttgataa aaatcatta                                                   139


<210>  21
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA-OUT antisense repressor 
       RNA

<400>  21
ttcgcacatc ttgttgtctg attattgatt tttggcgaaa ccatttgatc atatgacaag       60

atgtgtatct                                                              70


<210>  22
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free RNA-OUT selection 
       marker

<400>  22
gtagaattgg taaagagagt tgtgtaaaat attgagttag cacatcttgt tgtctgatta       60

ttgatttttg gggaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg      120

attttgataa aaatcatta                                                   139


<210>  23
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA-OUT-ColE2 origin 
       Bacterial region [NheI site-ssiA-ColE2 Origin (+7)-RNA-OUT-KpnI 
       site]

<400>  23
gctagctaca atggctcatg tggaaaaacc attggcagaa aaacacctgc caacagtttt       60

accacaattg ccacttaacc cacaaaaggg cgctgttatc tgataaggct tatctggtct      120

cattttgcac gttgtggtag aattggtaaa gagagtcgtg taaaatatcg agttcgcaca      180

tcttgttgtc tgattattga tttttggcga aaccatttga tcatatgaca agatgtgtat      240

ctaccttaac ttaatgattt tgataaaaat cattaggtac c                          281


<210>  24
<211>  275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C2 and NTC9385C2a 
       intronic Bacterial region [filled NheI site-ssiA-ColE2 Origin 
       (+7)-RNA-OUT-chewed KpnI site] Sequence shown is O1; O2 is 
       reverse complement

<400>  24
ctagctacaa tggctcatgt ggaaaaacca ttggcagaaa aacacctgcc aacagtttta       60

ccacaattgc cacttaaccc acaaaagggc gctgttatct gataaggctt atctggtctc      120

attttgcacg ttgtggtaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat      180

cttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc      240

taccttaact taatgatttt gataaaaatc attag                                 275


<210>  25
<211>  280
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free  ColE2 RNA-OUT 
       bacterial region. (CpG free ssiA-CpG free ColE2 origin-CpG free 
       RNA-OUT selection marker)- - flanked by SphI and BglII 
       restriction sites

<400>  25
agcatgctac aatggctcat gtggaaaaac cattggcaga aaaacacctg ccaacagttt       60

taccacaatt gccacttaac ccacaaaagg gggctgttat ctgataaggc ttatctggtc      120

tcattttggt acctggtaga attggtaaag agagttgtgt aaaatattga gttagcacat      180

cttgttgtct gattattgat ttttggggaa accatttgat catatgacaa gatgtgtatc      240

taccttaact taatgatttt gataaaaatc attaagatct                            280


<210>  26
<211>  466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA-OUT-R6K gamma origin 
       Bacterial region [NheI site-trpA terminator-R6K 
       Origin-RNA-OUT-KpnI site]

<400>  26
gctagcccgc ctaatgagcg ggcttttttt tggcttgttg tccacaaccg ttaaacctta       60

aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct      120

atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa      180

catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc      240

gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtacta tcaacaggtt      300

gaactgctga tccacgttgt ggtagaattg gtaaagagag tcgtgtaaaa tatcgagttc      360

gcacatcttg ttgtctgatt attgattttt ggcgaaacca tttgatcata tgacaagatg      420

tgtatctacc ttaacttaat gattttgata aaaatcatta ggtacc                     466


<210>  27
<211>  460
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R2 and NTC9385R2a 
       intronic R6K gamma origin- RNA-OUT Bacterial region [filled NheI 
       site-trpA terminator-R6K Origin-RNA-OUT-chewed KpnI site] 
       Sequence shown is O1; O2 is reverse complement

<400>  27
ctagcccgcc taatgagcgg gctttttttt ggcttgttgt ccacaaccgt taaaccttaa       60

aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta      120

tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac      180

atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg      240

ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtactat caacaggttg      300

aactgctgat ccacgttgtg gtagaattgg taaagagagt cgtgtaaaat atcgagttcg      360

cacatcttgt tgtctgatta ttgatttttg gcgaaaccat ttgatcatat gacaagatgt      420

gtatctacct taacttaatg attttgataa aaatcattag                            460


<210>  28
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free R6K gamma origin - 
       RNA-OUT bacterial region flanked by SphI and BglII restriction 
       sites

<400>  28
agcatgcaaa ccttaaaacc tttaaaagcc ttatatattc ttttttttct tataaaactt       60

aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga      120

gcttagtaca tgaaacatga gagcttagta cattagccat gagagcttag tacattagcc      180

atgagggttt agttcattaa acatgagagc ttagtacatt aaacatgaga gcttagtaca      240

tactatcaac aggttgaact gctgatcggt acctggtaga attggtaaag agagttgtgt      300

aaaatattga gttagcacat cttgttgtct gattattgat ttttggggaa accatttgat      360

catatgacaa gatgtgtatc taccttaact taatgatttt gataaaaatc attaagatct      420


<210>  29
<211>  1210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385P2 and NTC9385P2a 
       intronic pUC origin- RNA-OUT Bacterial region [filled NheI 
       site-trpA terminator-pUC Origin-RNA-OUT-chewed KpnI site] 
       Sequence shown is O1; O2 is reverse complement

<400>  29
ctagcccgcc taatgagcgg gctttttttt cttaggcctt cttccgcttc ctcgctcact       60

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta      120

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag      180

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc      240

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta      300

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg      360

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc      420

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac      480

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac      540

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg      600

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga      660

agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt      720

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag      780

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct      840

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg      900

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat      960

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     1020

tgtctatttc gttcatccat agttgcctga ctcctgcaaa ccacgttgtg gtagaattgg     1080

taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta ttgatttttg     1140

gcgaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg attttgataa     1200

aaatcattag                                                            1210


<210>  30
<211>  1196
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C2, NTC9385R2, 
       NTC9385P2 and NTC9385P2(0.85)  Eukaryotic region.  Bp 1 is start 
       of CMV enhancer, bp 1196  is end of polyadenylation site. Exon 2 
       encoded SalI (GTCGAC) and BglII (AGATCT) transgene cloning sites.

<400>  30
ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt       60

tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac      120

gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg      180

ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag      240

tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat      300

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat      360

ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt      420

tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga      480

ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg      540

gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca      600

tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggctcgca      660

tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg      720

ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa      780

agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc      840

ggctctccac gctttgcctg accctgcttg ctcaactcta gttctctcgt taacttaatg      900

agacagatag aaactggtct tgtagaaaca gagtagtcgc ctgcttttct gccaggtgct      960

gacttctctc ccctgggctt ttttcttttt ctcaggttga aaagaagaag acgaagaaga     1020

cgaagaagac aaaccgtcgt cgacagatct ttttccctct gccaaaaatt atggggacat     1080

catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat     1140

agtgtgttgg aattttttgt gtctctcact cggaaggaca taagggcggc cgctag         1196


<210>  31
<211>  1292
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C2a, NTC9385R2a, 
       NTC9385P2a and  NTC9385P2a(0.85) Eukaryotic region. Bp 1 start of
       CMV enhancer encoded boundary region, bp 1292 is end of 
       polyadenylation site. Exon 2 encoded SalI and BglII transgene 
       

<400>  31
atggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc       60

aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg      120

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      180

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      240

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      300

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      360

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      420

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat      480

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      540

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc      600

cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      660

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag      720

aagacaccgg gaccgatcca gcctccgcgg ctcgcatctc tccttcacgc gcccgccgcc      780

ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg      840

cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt      900

gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc      960

tgcttgctca actctagttc tctcgttaac ttaatgagac agatagaaac tggtcttgta     1020

gaaacagagt agtcgcctgc ttttctgcca ggtgctgact tctctcccct gggctttttt     1080

ctttttctca ggttgaaaag aagaagacga agaagacgaa gaagacaaac cgtcgtcgac     1140

agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac     1200

ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct     1260

ctcactcgga aggacataag ggcggccgct ag                                   1292


<210>  32
<211>  230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: CpG free HTLV- IR-Rabbit Beta
       globin hybrid intron

<400>  32
aggtaagttt aaagctcagg tcagagacca gggcctttgt ccaggcagct cccttggagc       60

ctacctagac tcagccaggc tctccagctt tgcctgaccc tgcttgctca actctagttc      120

tctgttaact taatgagaca gatagaaact ggtcttgtag aaacagagta gtgcctgctt      180

ttctgccagg tgctgacttc tctcccctgg gcttttttct ttttctcagg                 230


<210>  33
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNAI antisense repressor RNA 
       (pMB1 plasmid origin RNAII antisense partner)

<400>  33
acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc       60

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtt                   108


<210>  34
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNAI selectable Marker

<400>  34
ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg       60

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc      120

gctggtagcg gtggtttttt tgtttgcaag cagcag                                156


<210>  35
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: IncB RNAI antisense repressor
       RNA (IncB plasmid origin RNAII antisense partner)

<400>  35
gtattctgtg aggcccccat tatttttctg cgttccgcca agttcgagga aaaatagtgg       60

gggttttcct tta                                                          73


<210>  36
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: IncB RNAI selectable Marker, 
       RNAI  RNA (Sense strand).

<400>  36
ttgaatctct ggtacggttt catatatact tatcccgtat tctgtgaggc ccccattatt       60

tttctgcgtt ccgccaagtt cgaggaaaaa tagtgggggt tttccttta                  109


<210>  37
<211>  362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: IncB RNAII-SacB. PstI-MamI 
       restriction fragment

<400>  37
ctgcagttca aagcggtgga aaaggggtat attgcggatc gttattcagt ggcttttggg       60

atcctcgcgg tccggaaagc cagaaaacgg cagaatgcgc cataaggcat tcaggacgta      120

tggcagaaac gacggcagtt tgccggtgcc ggaaggctga aaaaagtttc agaagaccat      180

aaaggaaaac ccccactatt tttcctcgaa cttggcggaa cgcagaaaaa taatgggggc      240

ctcacagaat acgggatagg gcccatgaaa ccgtaccaga gattgggccc tgtgcagtgt      300

ataaatacac ggcacaatcg ctccgccata agcgacagct tgtggcaggt ctgatgaaca      360

tc                                                                     362


<210>  38
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA selection marker (RSM) 
       antisense repressor RNA

<400>  38
atgttcatgt tcttgtctcc ttattgattt ttggggaaac catttgatca tatgagaaca       60

tgaacta                                                                 67


<210>  39
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA selectable marker (RSM)

<400>  39
gttgacatgg taaagagagt tgtgtaaaat attgagtatg ttcatgttct tgtctcctta       60

ttgatttttg gggaaaccat ttgatcatat gagaacatga actactacct taacttaatg      120

attttgataa aaatcatta                                                   139


<210>  40
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RSM complement

<400>  40
cccaaaaatc aataaggaga caagaacatg aacat                                  35


<210>  41
<211>  1497
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA selection-sacB (P5/6 4/6)
       PstI/BamHI restriction fragment

<400>  41
ctgcaggtag acatttatcc cttgcggcga tagattggtt tccccaaaaa tcaataagga       60

gacaagaaca tgaacatcaa aaaattcgcc aaacaggcaa ccgtcttgac cttcaccacc      120

gcactgctgg caggcggtgc aacccaggca tttgcaaaag aaaccaatca gaagccgtac      180

aaagaaacct atggcattag ccacatcaca cgccatgaca tgctgcaaat tccggaacag      240

cagaaaaacg agaagtacaa agtgccggaa ttcgatagca gcaccattaa aaacattagc      300

agcgcaaaag gtctggatgt ttgggatagc tggcctctgc aaaataccga tggcaccgtt      360

gcaaattacc acggctatca cattgttttt gcactggctg gtgatccgaa aaatgcagac      420

gacaccagca tctacatgtt ttatcagaaa gttggcgaaa ccagcatcga cagctggaag      480

aacgccggtc gtgtttttaa agatagcgat aaatttgatg ccaacgatag catcctgaaa      540

gatcagaccc aagagtggtc tggtagcgcc acctttacct cagatggcaa gattcgtctg      600

ttctacaccg atttcagcgg taaacattat ggtaaacaga ccttgaccac agcccaggtt      660

aacgtcagcg caagcgatag cagtctgaat attaacggtg tggaggacta caaaagcatc      720

tttgatggtg atggcaaaac ctatcagaac gtgcagcagt ttattgatga aggcaattac      780

agcagcggtg acaaccatac cctgcgtgat ccgcattatg ttgaagataa aggccataaa      840

tatctggtgt ttgaagcaaa taccggcacc gaagacggtt atcagggtga agaaagcctg      900

tttaacaaag cctactacgg caaaagcacc agcttttttc gccaagaaag ccagaaactg      960

ctgcaaagcg acaagaaacg taccgcagaa ctggcaaatg gtgcactggg catgattgaa     1020

ctgaatgatg actacaccct gaaaaaagtg atgaaaccgc tgattgcaag caataccgtt     1080

accgacgaga ttgaacgtgc caacgtgttc aagatgaacg gtaagtggta cctgtttacc     1140

gatagccgtg gtagcaaaat gaccattgat ggtattacga gcaacgatat ttacatgctg     1200

ggttacgtca gcaatagctt aaccggtccg tacaaacctc tgaataaaac cggtctggtt     1260

ctgaaaatgg atctggaccc gaatgacgtc accttcacct attcacactt cgcagttccg     1320

caggccaaag gcaataacgt tgttatcact agctacatga ccaaccgtgg tttctacgca     1380

gataaacaga gcacctttgc accgagcttt ctgctgaaca ttaaaggtaa aaaaaccagc     1440

gtggtgaaag acagcatcct ggaacagggc cagcttaccg tgaacaagtg aggatcc        1497


<210>  42
<211>  1497
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNA selection-sacB (P5/6 5/6)
       PstI/BamHI restriction fragment

<400>  42
ctgcaggtag acacacatct tgtcatatga tagaatggtt tccccaaaaa tcaataagga       60

gacaagaaca tgaacatcaa aaaattcgcc aaacaggcaa ccgtcttgac cttcaccacc      120

gcactgctgg caggcggtgc aacccaggca tttgcaaaag aaaccaatca gaagccgtac      180

aaagaaacct atggcattag ccacatcaca cgccatgaca tgctgcaaat tccggaacag      240

cagaaaaacg agaagtacaa agtgccggaa ttcgatagca gcaccattaa aaacattagc      300

agcgcaaaag gtctggatgt ttgggatagc tggcctctgc aaaataccga tggcaccgtt      360

gcaaattacc acggctatca cattgttttt gcactggctg gtgatccgaa aaatgcagac      420

gacaccagca tctacatgtt ttatcagaaa gttggcgaaa ccagcatcga cagctggaag      480

aacgccggtc gtgtttttaa agatagcgat aaatttgatg ccaacgatag catcctgaaa      540

gatcagaccc aagagtggtc tggtagcgcc acctttacct cagatggcaa gattcgtctg      600

ttctacaccg atttcagcgg taaacattat ggtaaacaga ccttgaccac agcccaggtt      660

aacgtcagcg caagcgatag cagtctgaat attaacggtg tggaggacta caaaagcatc      720

tttgatggtg atggcaaaac ctatcagaac gtgcagcagt ttattgatga aggcaattac      780

agcagcggtg acaaccatac cctgcgtgat ccgcattatg ttgaagataa aggccataaa      840

tatctggtgt ttgaagcaaa taccggcacc gaagacggtt atcagggtga agaaagcctg      900

tttaacaaag cctactacgg caaaagcacc agcttttttc gccaagaaag ccagaaactg      960

ctgcaaagcg acaagaaacg taccgcagaa ctggcaaatg gtgcactggg catgattgaa     1020

ctgaatgatg actacaccct gaaaaaagtg atgaaaccgc tgattgcaag caataccgtt     1080

accgacgaga ttgaacgtgc caacgtgttc aagatgaacg gtaagtggta cctgtttacc     1140

gatagccgtg gtagcaaaat gaccattgat ggtattacga gcaacgatat ttacatgctg     1200

ggttacgtca gcaatagctt aaccggtccg tacaaacctc tgaataaaac cggtctggtt     1260

ctgaaaatgg atctggaccc gaatgacgtc accttcacct attcacactt cgcagttccg     1320

caggccaaag gcaataacgt tgttatcact agctacatga ccaaccgtgg tttctacgca     1380

gataaacaga gcacctttgc accgagcttt ctgctgaaca ttaaaggtaa aaaaaccagc     1440

gtggtgaaag acagcatcct ggaacagggc cagcttaccg tgaacaagtg aggatcc        1497


<210>  43
<211>  4015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: pINT-RNAS integration vector 
       (P5/6 4/6)

<400>  43
ctgcaggtag acatttatcc cttgcggcga tagattggtt tccccaaaaa tcaataagga       60

gacaagaaca tgaacatcaa aaaattcgcc aaacaggcaa ccgtcttgac cttcaccacc      120

gcactgctgg caggcggtgc aacccaggca tttgcaaaag aaaccaatca gaagccgtac      180

aaagaaacct atggcattag ccacatcaca cgccatgaca tgctgcaaat tccggaacag      240

cagaaaaacg agaagtacaa agtgccggaa ttcgatagca gcaccattaa aaacattagc      300

agcgcaaaag gtctggatgt ttgggatagc tggcctctgc aaaataccga tggcaccgtt      360

gcaaattacc acggctatca cattgttttt gcactggctg gtgatccgaa aaatgcagac      420

gacaccagca tctacatgtt ttatcagaaa gttggcgaaa ccagcatcga cagctggaag      480

aacgccggtc gtgtttttaa agatagcgat aaatttgatg ccaacgatag catcctgaaa      540

gatcagaccc aagagtggtc tggtagcgcc acctttacct cagatggcaa gattcgtctg      600

ttctacaccg atttcagcgg taaacattat ggtaaacaga ccttgaccac agcccaggtt      660

aacgtcagcg caagcgatag cagtctgaat attaacggtg tggaggacta caaaagcatc      720

tttgatggtg atggcaaaac ctatcagaac gtgcagcagt ttattgatga aggcaattac      780

agcagcggtg acaaccatac cctgcgtgat ccgcattatg ttgaagataa aggccataaa      840

tatctggtgt ttgaagcaaa taccggcacc gaagacggtt atcagggtga agaaagcctg      900

tttaacaaag cctactacgg caaaagcacc agcttttttc gccaagaaag ccagaaactg      960

ctgcaaagcg acaagaaacg taccgcagaa ctggcaaatg gtgcactggg catgattgaa     1020

ctgaatgatg actacaccct gaaaaaagtg atgaaaccgc tgattgcaag caataccgtt     1080

accgacgaga ttgaacgtgc caacgtgttc aagatgaacg gtaagtggta cctgtttacc     1140

gatagccgtg gtagcaaaat gaccattgat ggtattacga gcaacgatat ttacatgctg     1200

ggttacgtca gcaatagctt aaccggtccg tacaaacctc tgaataaaac cggtctggtt     1260

ctgaaaatgg atctggaccc gaatgacgtc accttcacct attcacactt cgcagttccg     1320

caggccaaag gcaataacgt tgttatcact agctacatga ccaaccgtgg tttctacgca     1380

gataaacaga gcacctttgc accgagcttt ctgctgaaca ttaaaggtaa aaaaaccagc     1440

gtggtgaaag acagcatcct ggaacagggc cagcttaccg tgaacaagtg aggatccccg     1500

gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc gatgacgcat     1560

cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt acaaagcgag     1620

gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag cggctggctg     1680

aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg agttaagaac     1740

gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc caataccagt     1800

agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgtgct tctctggagt     1860

gcgacaggtt tgatgacaaa aaattagcgc aagaagacaa aaatcacctt gcgctaatgc     1920

tctgttacag gtcactaata ccatctaagt agttgattca tagtgactgc atatatgttg     1980

tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt     2040

tatatcattt tacgtttctc gttcagcttt tttatactaa gttggcatta taaaaaagca     2100

ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg     2160

atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc catggctaat     2220

tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg     2280

gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt     2340

aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt     2400

tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag     2460

cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca     2520

tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt     2580

actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg     2640

atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     2700

aacttggtct gacagttacc aatgcttaat cagtgaggca ccaataactg ccttaaaaaa     2760

attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga     2820

catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt     2880

cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg     2940

ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat     3000

tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg     3060

aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg     3120

tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct     3180

caccgtcttt cattgccata cggaatttcg gatgagcatt catcaggcgg gcaagaatgt     3240

gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa     3300

tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat     3360

gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca     3420

ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc     3480

ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc     3540

aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga     3600

agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact     3660

tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc     3720

atcgatggcc cccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc     3780

atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt     3840

cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc     3900

aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc     3960

agaaggccat cctgacggat ggcctttttg cgtggccagt gccaagcttg catgc          4015


<210>  44
<211>  4015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: pINT-RNAS integration vector 
       (P5/6 5/6)

<400>  44
ctgcaggtag acacacatct tgtcatatga tagaatggtt tccccaaaaa tcaataagga       60

gacaagaaca tgaacatcaa aaaattcgcc aaacaggcaa ccgtcttgac cttcaccacc      120

gcactgctgg caggcggtgc aacccaggca tttgcaaaag aaaccaatca gaagccgtac      180

aaagaaacct atggcattag ccacatcaca cgccatgaca tgctgcaaat tccggaacag      240

cagaaaaacg agaagtacaa agtgccggaa ttcgatagca gcaccattaa aaacattagc      300

agcgcaaaag gtctggatgt ttgggatagc tggcctctgc aaaataccga tggcaccgtt      360

gcaaattacc acggctatca cattgttttt gcactggctg gtgatccgaa aaatgcagac      420

gacaccagca tctacatgtt ttatcagaaa gttggcgaaa ccagcatcga cagctggaag      480

aacgccggtc gtgtttttaa agatagcgat aaatttgatg ccaacgatag catcctgaaa      540

gatcagaccc aagagtggtc tggtagcgcc acctttacct cagatggcaa gattcgtctg      600

ttctacaccg atttcagcgg taaacattat ggtaaacaga ccttgaccac agcccaggtt      660

aacgtcagcg caagcgatag cagtctgaat attaacggtg tggaggacta caaaagcatc      720

tttgatggtg atggcaaaac ctatcagaac gtgcagcagt ttattgatga aggcaattac      780

agcagcggtg acaaccatac cctgcgtgat ccgcattatg ttgaagataa aggccataaa      840

tatctggtgt ttgaagcaaa taccggcacc gaagacggtt atcagggtga agaaagcctg      900

tttaacaaag cctactacgg caaaagcacc agcttttttc gccaagaaag ccagaaactg      960

ctgcaaagcg acaagaaacg taccgcagaa ctggcaaatg gtgcactggg catgattgaa     1020

ctgaatgatg actacaccct gaaaaaagtg atgaaaccgc tgattgcaag caataccgtt     1080

accgacgaga ttgaacgtgc caacgtgttc aagatgaacg gtaagtggta cctgtttacc     1140

gatagccgtg gtagcaaaat gaccattgat ggtattacga gcaacgatat ttacatgctg     1200

ggttacgtca gcaatagctt aaccggtccg tacaaacctc tgaataaaac cggtctggtt     1260

ctgaaaatgg atctggaccc gaatgacgtc accttcacct attcacactt cgcagttccg     1320

caggccaaag gcaataacgt tgttatcact agctacatga ccaaccgtgg tttctacgca     1380

gataaacaga gcacctttgc accgagcttt ctgctgaaca ttaaaggtaa aaaaaccagc     1440

gtggtgaaag acagcatcct ggaacagggc cagcttaccg tgaacaagtg aggatccccg     1500

gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc gatgacgcat     1560

cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt acaaagcgag     1620

gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag cggctggctg     1680

aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg agttaagaac     1740

gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc caataccagt     1800

agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgtgct tctctggagt     1860

gcgacaggtt tgatgacaaa aaattagcgc aagaagacaa aaatcacctt gcgctaatgc     1920

tctgttacag gtcactaata ccatctaagt agttgattca tagtgactgc atatatgttg     1980

tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt     2040

tatatcattt tacgtttctc gttcagcttt tttatactaa gttggcatta taaaaaagca     2100

ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg     2160

atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc catggctaat     2220

tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg     2280

gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt     2340

aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt     2400

tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag     2460

cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca     2520

tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt     2580

actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg     2640

atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     2700

aacttggtct gacagttacc aatgcttaat cagtgaggca ccaataactg ccttaaaaaa     2760

attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga     2820

catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt     2880

cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg     2940

ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat     3000

tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg     3060

aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg     3120

tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct     3180

caccgtcttt cattgccata cggaatttcg gatgagcatt catcaggcgg gcaagaatgt     3240

gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa     3300

tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat     3360

gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca     3420

ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc     3480

ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc     3540

aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga     3600

agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact     3660

tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc     3720

atcgatggcc cccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc     3780

atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt     3840

cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc     3900

aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc     3960

agaaggccat cctgacggat ggcctttttg cgtggccagt gccaagcttg catgc          4015


<210>  45
<211>  678
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Pmin pUC replication origin 
       (minimal)

<400>  45
cgcgttgctg gcgtttttca taggctccgc ccccctgacg agcatcacaa aaatcgacgc       60

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga      120

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt      180

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg      240

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc      300

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg      360

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc      420

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg      480

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc      540

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct      600

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt      660

taagggattt tggtcatg                                                    678


<210>  46
<211>  860
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NTC9385P2(0.85) and NTC9385P2a(0.85) intronic pUC (0.85) 
       Bacterial region [filled NheI site-trpA terminator- Pmin pUC 
       replication origin-RNA-OUT-chewed KpnI site] Sequence shown is 
       O1; O2 is

<400>  46
ctagcccgcc taatgagcgg gctttttttt cttaggcctc gcgttgctgg cgtttttcat       60

aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac      120

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct      180

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg      240

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg      300

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt      360

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg      420

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac      480

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga      540

aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt      600

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt      660

tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatggtg      720

gtagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta      780

ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg      840

attttgataa aaatcattag                                                  860


<210>  47
<211>  1751
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RbF vector backbone

<400>  47
atggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc       60

aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg      120

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      180

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      240

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      300

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      360

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      420

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat      480

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      540

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc      600

cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      660

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag      720

aagacaccgg gaccgatcca gcctccgcgg ctcgcatctc tccttcacgc gcccgccgcc      780

ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg      840

cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt      900

gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc      960

tgcttgctca actctagttc tctcgttgtg gtagaattgg taaagagagt cgtgtaaaat     1020

atcgagttcg cacatcttgt tgtctgatta ttgatttttg gcgaaaccat ttgatcatat     1080

gacaagatgt gtatctacct taacttaatg attttgataa aaatcattag aacttaatga     1140

gacagataga aactggtctt gtagaaacag agtagtcgcc tgcttttctg ccaggtgctg     1200

acttctctcc cctgggcttt tttctttttc tcaggttgaa aagaagaaga cgaagaagac     1260

gaagaagaca aaccgtcgtc gacagatcta gcccgcctaa tgagcgggct tttttttggc     1320

ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc     1380

ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt     1440

caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta     1500

gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt taaacatgag     1560

agcttagtac gtactatcaa caggttgaac tgctgatcca catctttttc cctctgccaa     1620

aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt     1680

tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacataagg     1740

gcggccgcta g                                                          1751


<210>  48
<211>  1751
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RbF-RSM vector 
       backbone

<400>  48
atggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc       60

aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg      120

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      180

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      240

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      300

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      360

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      420

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat      480

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      540

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc      600

cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      660

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag      720

aagacaccgg gaccgatcca gcctccgcgg ctcgcatctc tccttcacgc gcccgccgcc      780

ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg      840

cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt      900

gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc      960

tgcttgctca actctagttc tctcgttgtg gttgacatgg taaagagagt tgtgtaaaat     1020

attgagtatg ttcatgttct tgtctcctta ttgatttttg gggaaaccat ttgatcatat     1080

gagaacatga actactacct taacttaatg attttgataa aaatcattag aacttaatga     1140

gacagataga aactggtctt gtagaaacag agtagtcgcc tgcttttctg ccaggtgctg     1200

acttctctcc cctgggcttt tttctttttc tcaggttgaa aagaagaaga cgaagaagac     1260

gaagaagaca aaccgtcgtc gacagatcta gcccgcctaa tgagcgggct tttttttggc     1320

ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc     1380

ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt     1440

caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta     1500

gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt taaacatgag     1560

agcttagtac gtactatcaa caggttgaac tgctgatcca catctttttc cctctgccaa     1620

aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt     1680

tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacataagg     1740

gcggccgcta g                                                          1751


<210>  49
<211>  1770
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RbF-RNAI vector 
       backbone

<400>  49
atggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc       60

aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg      120

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      180

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      240

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      300

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga      360

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg      420

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat      480

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      540

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc      600

cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc      660

tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag      720

aagacaccgg gaccgatcca gcctccgcgg ctcgcatctc tccttcacgc gcccgccgcc      780

ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg      840

cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt      900

gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc      960

tgcttgctca actctagttc tctcgttaac ttgaagtggt ggcctaacta cggctacact     1020

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     1080

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     1140

cagcaggtta acttaatgag acagatagaa actggtcttg tagaaacaga gtagtcgcct     1200

gcttttctgc caggtgctga cttctctccc ctgggctttt ttctttttct caggttgaaa     1260

agaagaagac gaagaagacg aagaagacaa accgtcgtcg acagatctag cccgcctaat     1320

gagcgggctt ttttttggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc     1380

ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat     1440

ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta     1500

cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc     1560

ttagtacgtt aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatccac     1620

atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt     1680

ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct     1740

cactcggaag gacataaggg cggccgctag                                      1770


<210>  50
<211>  1660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O1 vector backbone

<400>  50
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tgtggtagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc     1260

ttgttgtctg attattgatt tttggcgaaa ccatttgatc atatgacaag atgtgtatct     1320

accttaactt aatgattttg ataaaaatca ttagaactta atgagacaga tagaaactgg     1380

tcttgtagaa acagagtagt cgcctgcttt tctgccaggt gctgacttct ctcccctggg     1440

cttttttctt tttctcaggt tgaaaagaag aagacgaaga agacgaagaa gacaaaccgt     1500

cgtcgacaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc     1560

atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt     1620

tgtgtctctc actcggaagg acataagggc ggccgctagc                           1660


<210>  51
<211>  1660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O2 vector backbone

<400>  51
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt     1260

catatgatca aatggtttcg ccaaaaatca ataatcagac aacaagatgt gcgaactcga     1320

tattttacac gactctcttt accaattcta ccacaactta atgagacaga tagaaactgg     1380

tcttgtagaa acagagtagt cgcctgcttt tctgccaggt gctgacttct ctcccctggg     1440

cttttttctt tttctcaggt tgaaaagaag aagacgaaga agacgaagaa gacaaaccgt     1500

cgtcgacaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc     1560

atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt     1620

tgtgtctctc actcggaagg acataagggc ggccgctagc                           1660


<210>  52
<211>  1660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O1-RSM vector 
       backbone

<400>  52
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tgtggttgac atggtaaaga gagttgtgta aaatattgag tatgttcatg     1260

ttcttgtctc cttattgatt tttggggaaa ccatttgatc atatgagaac atgaactact     1320

accttaactt aatgattttg ataaaaatca ttagaactta atgagacaga tagaaactgg     1380

tcttgtagaa acagagtagt cgcctgcttt tctgccaggt gctgacttct ctcccctggg     1440

cttttttctt tttctcaggt tgaaaagaag aagacgaaga agacgaagaa gacaaaccgt     1500

cgtcgacaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc     1560

atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt     1620

tgtgtctctc actcggaagg acataagggc ggccgctagc                           1660


<210>  53
<211>  1660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O2-RSM  vector 
       backbone

<400>  53
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tctaatgatt tttatcaaaa tcattaagtt aaggtagtag ttcatgttct     1260

catatgatca aatggtttcc ccaaaaatca ataaggagac aagaacatga acatactcaa     1320

tattttacac aactctcttt accatgtcaa ccacaactta atgagacaga tagaaactgg     1380

tcttgtagaa acagagtagt cgcctgcttt tctgccaggt gctgacttct ctcccctggg     1440

cttttttctt tttctcaggt tgaaaagaag aagacgaaga agacgaagaa gacaaaccgt     1500

cgtcgacaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc     1560

atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt     1620

tgtgtctctc actcggaagg acataagggc ggccgctagc                           1660


<210>  54
<211>  1673
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O1-RNAI vector 
       backbone

<400>  54
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta     1260

tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca     1320

aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagaac ttaatgagac     1380

agatagaaac tggtcttgta gaaacagagt agtcgcctgc ttttctgcca ggtgctgact     1440

tctctcccct gggctttttt ctttttctca ggttgaaaag aagaagacga agaagacgaa     1500

gaagacaaac cgtcgtcgac agatcttttt ccctctgcca aaaattatgg ggacatcatg     1560

aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg     1620

tgttggaatt ttttgtgtct ctcactcgga aggacataag ggcggccgct agc            1673


<210>  55
<211>  1673
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385Ra-O2-RNAI  vector 
       backbone

<400>  55
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     1260

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     1320

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaaaac ttaatgagac     1380

agatagaaac tggtcttgta gaaacagagt agtcgcctgc ttttctgcca ggtgctgact     1440

tctctcccct gggctttttt ctttttctca ggttgaaaag aagaagacga agaagacgaa     1500

gaagacaaac cgtcgtcgac agatcttttt ccctctgcca aaaattatgg ggacatcatg     1560

aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg     1620

tgttggaatt ttttgtgtct ctcactcgga aggacataag ggcggccgct agc            1673


<210>  56
<211>  1665
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RaF  vector backbone

<400>  56
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt taacttaatg agacagatag aaactggtct tgtagaaaca gagtagtcgc     1260

ctgcttttct gccaggtgct gacttctctc ccctgggctt ttttcttttt ctcaggttga     1320

aaagaagaag acgaagaaga cgaagaagac aaaccgtcgt cgacagatct gtggtagaat     1380

tggtaaagag agtcgtgtaa aatatcgagt tcgcacatct tgttgtctga ttattgattt     1440

ttggcgaaac catttgatca tatgacaaga tgtgtatcta ccttaactta atgattttga     1500

taaaaatcat taggatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct     1560

tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa     1620

ttttttgtgt ctctcactcg gaaggacata agggcggccg ctagc                     1665


<210>  57
<211>  1665
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RaF-RSM  vector 
       backbone

<400>  57
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt taacttaatg agacagatag aaactggtct tgtagaaaca gagtagtcgc     1260

ctgcttttct gccaggtgct gacttctctc ccctgggctt ttttcttttt ctcaggttga     1320

aaagaagaag acgaagaaga cgaagaagac aaaccgtcgt cgacagatct gtggttgaca     1380

tggtaaagag agttgtgtaa aatattgagt atgttcatgt tcttgtctcc ttattgattt     1440

ttggggaaac catttgatca tatgagaaca tgaactacta ccttaactta atgattttga     1500

taaaaatcat taggatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct     1560

tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa     1620

ttttttgtgt ctctcactcg gaaggacata agggcggccg ctagc                     1665


<210>  58
<211>  1695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385RaF -RNAI  vector 
       backbone

<400>  58
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacc ccggctctag ttattaatag taatcaatta cggggtcatt agttcatagc      360

ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc      420

aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg      480

actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat      540

caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc      600

tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta      660

ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag      720

cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt      780

tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa      840

atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt      900

cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga      960

tccagcctcc gcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca     1020

tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc     1080

cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg     1140

gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta     1200

gttctctcgt taacttaatg agacagatag aaactggtct tgtagaaaca gagtagtcgc     1260

ctgcttttct gccaggtgct gacttctctc ccctgggctt ttttcttttt ctcaggttga     1320

aaagaagaag acgaagaaga cgaagaagac aaaccgtcgt cgacagatct accgttaacc     1380

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag     1440

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt     1500

cttctagtgt agccgtagtt aggccaccac ttcaagttaa cacgatcttt ttccctctgc     1560

caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa     1620

atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata     1680

agggcggccg ctagc                                                      1695


<210>  59
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Anti-RNAI (10-108)

<400>  59
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct       60

ttttccgaag gtaactggct tcagcagagc gcagatacc                              99


<210>  60
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Anti-RNAI (10-108)-weak 
       RBS-ATG

<400>  60
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct       60

ttttccgaag gtaactggct tcagcagagc gcagataccg tcgacaagaa catg            114


<210>  61
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Anti-RNAI (10-108)-strong 
       RBS-ATG

<400>  61
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct       60

ttttccgaag gtaactggct tcagcagagc gcagataccg tcgacaggag acaagaacat      120

g                                                                      121


<210>  62
<211>  1667
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R vector backbone. Bp 
       1 is start of trpA terminator upstream of R6K origin, last bp  is
       end of polyadenylation signal. Exon 2 encoded SalI (GTCGAC) and 
       BglII (AGATCT) transgene cloning sites are juxtaposed

<400>  62
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtggtaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat      360

cttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc      420

taccttaact taatgatttt gataaaaatc attaggtacc ccggctctag ttattaatag      480

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt      540

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg      600

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat      660

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct      720

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg      780

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg      840

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc      900

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa      960

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     1020

tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt     1080

tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggctcgca tctctccttc     1140

acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc     1200

tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc     1260

gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac     1320

gctttgcctg accctgcttg ctcaactcta gttctctcgt taacttaatg agacagatag     1380

aaactggtct tgtagaaaca gagtagtcgc ctgcttttct gccaggtgct gacttctctc     1440

ccctgggctt ttttcttttt ctcaggttga aaagaagaag acgaagaaga cgaagaagac     1500

aaaccgtcgt cgacagatct ttttccctct gccaaaaatt atggggacat catgaagccc     1560

cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg     1620

aattttttgt gtctctcact cggaaggaca taagggcggc cgctagc                   1667


<210>  63
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C vector backbone. Bp 
       1 is start of ssiA upstream of ColE2 origin, last bp  is end of 
       polyadenylation signal. Exon 2 encoded SalI (GTCGAC) and BglII 
       (AGATCT) transgene cloning sites are juxtaposed

<400>  63
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg gtagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt      180

tgtctgatta ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct      240

taacttaatg attttgataa aaatcattag gtaccccggc tctagttatt aatagtaatc      300

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt      360

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta      420

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg      480

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga      540

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt      600

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg      660

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc      720

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg      780

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat      840

aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga      900

cctccataga agacaccggg accgatccag cctccgcggc tcgcatctct ccttcacgcg      960

cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg     1020

cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac     1080

cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt     1140

gcctgaccct gcttgctcaa ctctagttct ctcgttaact taatgagaca gatagaaact     1200

ggtcttgtag aaacagagta gtcgcctgct tttctgccag gtgctgactt ctctcccctg     1260

ggcttttttc tttttctcag gttgaaaaga agaagacgaa gaagacgaag aagacaaacc     1320

gtcgtcgaca gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga     1380

gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt     1440

tttgtgtctc tcactcggaa ggacataagg gcggccgcta gc                        1482


<210>  64
<211>  1486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R-intron vector 
       backbone. Bp 1 is start of trpA terminator upstream of R6K 
       origin, last bp  is end of polyadenylation signal. Exon 2 encoded
       SalI (GTCGAC) and BglII (AGATCT) transgene cloning sites are 
       

<400>  64
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtggtaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat      360

cttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc      420

taccttaact taatgatttt gataaaaatc attaggtacc ccggctctag ttattaatag      480

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt      540

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg      600

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat      660

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct      720

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg      780

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg      840

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc      900

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa      960

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     1020

tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt     1080

tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt     1140

ggaacgcgga ttccccgtgc caagagtcag gtaagtagtt aacttaatga gacagataga     1200

aactggtctt gtagaaacag agtagtcgcc tgcttttctg ccaggtgctg acttctctcc     1260

cctgggcttt tttctttttc tcaggttgaa aagaagaaga cgaagaagac gaagaagaca     1320

aaccgtcgtc gacagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc     1380

ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga     1440

attttttgtg tctctcactc ggaaggacat aagggcggcc gctagc                    1486


<210>  65
<211>  1486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R-intron RSM vector 
       backbone. Bp 1 is start of trpA terminator upstream of R6K 
       origin, last bp  is end of polyadenylation signal. Exon 2 encoded
       SalI (GTCGAC) and BglII (AGATCT) transgene cloning sites are 
       

<400>  65
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtggttga catggtaaag agagttgtgt aaaatattga gtatgttcat      360

gttcttgtct ccttattgat ttttggggaa accatttgat catatgagaa catgaactac      420

taccttaact taatgatttt gataaaaatc attaggtacc ccggctctag ttattaatag      480

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt      540

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg      600

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat      660

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct      720

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg      780

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg      840

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc      900

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa      960

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     1020

tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt     1080

tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt     1140

ggaacgcgga ttccccgtgc caagagtcag gtaagtagtt aacttaatga gacagataga     1200

aactggtctt gtagaaacag agtagtcgcc tgcttttctg ccaggtgctg acttctctcc     1260

cctgggcttt tttctttttc tcaggttgaa aagaagaaga cgaagaagac gaagaagaca     1320

aaccgtcgtc gacagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc     1380

ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga     1440

attttttgtg tctctcactc ggaaggacat aagggcggcc gctagc                    1486


<210>  66
<211>  1514
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R-intron RNAI vector 
       backbone. Bp 1 is start of trpA terminator upstream of R6K 
       origin, last bp  is end of polyadenylation signal. Exon 2 encoded
       SalI (GTCGAC) and BglII (AGATCT) transgene cloning sites are 
       

<400>  66
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtgttaac ttgaagtggt ggcctaacta cggctacact agaagaacag      360

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt      420

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcaggtta      480

acggtacccc ggctctagtt attaatagta atcaattacg gggtcattag ttcatagccc      540

atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa      600

cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac      660

tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca      720

agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg      780

gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt      840

agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg      900

gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg      960

gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat     1020

gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca     1080

gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc     1140

cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtcaggt     1200

aagtagttaa cttaatgaga cagatagaaa ctggtcttgt agaaacagag tagtcgcctg     1260

cttttctgcc aggtgctgac ttctctcccc tgggcttttt tctttttctc aggttgaaaa     1320

gaagaagacg aagaagacga agaagacaaa ccgtcgtcga cagatctttt tccctctgcc     1380

aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa     1440

tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacataa     1500

gggcggccgc tagc                                                       1514


<210>  67
<211>  1301
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C-intron vector 
       backbone. Bp 1 is start of ssiA upstream of ColE2 origin, last bp
       is end of polyadenylation signal. Exon 2 encoded SalI (GTCGAC) 
       and BglII (AGATCT) transgene cloning sites are juxtaposed

<400>  67
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg gtagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt      180

tgtctgatta ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct      240

taacttaatg attttgataa aaatcattag gtaccccggc tctagttatt aatagtaatc      300

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt      360

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta      420

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg      480

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga      540

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt      600

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg      660

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc      720

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg      780

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat      840

aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga      900

cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac      960

gcggattccc cgtgccaaga gtcaggtaag tagttaactt aatgagacag atagaaactg     1020

gtcttgtaga aacagagtag tcgcctgctt ttctgccagg tgctgacttc tctcccctgg     1080

gcttttttct ttttctcagg ttgaaaagaa gaagacgaag aagacgaaga agacaaaccg     1140

tcgtcgacag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag     1200

catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt     1260

ttgtgtctct cactcggaag gacataaggg cggccgctag c                         1301


<210>  68
<211>  1301
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C-intron RSM vector 
       backbone. Bp 1 is start of ssiA upstream of ColE2 origin, last bp
       is end of polyadenylation signal. Exon 2 encoded SalI (GTCGAC) 
       and BglII (AGATCT) transgene cloning sites are juxtaposed

<400>  68
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg gttgacatgg taaagagagt tgtgtaaaat attgagtatg ttcatgttct      180

tgtctcctta ttgatttttg gggaaaccat ttgatcatat gagaacatga actactacct      240

taacttaatg attttgataa aaatcattag gtaccccggc tctagttatt aatagtaatc      300

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt      360

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta      420

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg      480

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga      540

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt      600

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg      660

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc      720

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg      780

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat      840

aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga      900

cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac      960

gcggattccc cgtgccaaga gtcaggtaag tagttaactt aatgagacag atagaaactg     1020

gtcttgtaga aacagagtag tcgcctgctt ttctgccagg tgctgacttc tctcccctgg     1080

gcttttttct ttttctcagg ttgaaaagaa gaagacgaag aagacgaaga agacaaaccg     1140

tcgtcgacag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag     1200

catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt     1260

ttgtgtctct cactcggaag gacataaggg cggccgctag c                         1301


<210>  69
<211>  1329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C-intron RNAI vector 
       backbone. Bp 1 is start of ssiA upstream of ColE2 origin, last bp
       is end of polyadenylation signal. Exon 2 encoded SalI (GTCGAC) 
       and BglII (AGATCT) transgene cloning sites are juxtaposed

<400>  69
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg ttaacttgaa gtggtggcct aactacggct acactagaag aacagtattt      180

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc      240

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca ggttaacggt      300

accccggctc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      360

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      420

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      480

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      540

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      600

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      660

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      720

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      780

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      840

gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg      900

cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc      960

tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt caggtaagta     1020

gttaacttaa tgagacagat agaaactggt cttgtagaaa cagagtagtc gcctgctttt     1080

ctgccaggtg ctgacttctc tcccctgggc ttttttcttt ttctcaggtt gaaaagaaga     1140

agacgaagaa gacgaagaag acaaaccgtc gtcgacagat ctttttccct ctgccaaaaa     1200

ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat     1260

tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga cataagggcg     1320

gccgctagc                                                             1329


<210>  70
<211>  466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RSM-R6K gamma origin 
       bacterial region. [NheI site-trpA terminator-R6K Origin-RSM-KpnI 
       site]

<400>  70
gctagcccgc ctaatgagcg ggcttttttt tggcttgttg tccacaaccg ttaaacctta       60

aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct      120

atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa      180

catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc      240

gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtacta tcaacaggtt      300

gaactgctga tccacgttgt ggttgacatg gtaaagagag ttgtgtaaaa tattgagtat      360

gttcatgttc ttgtctcctt attgattttt ggggaaacca tttgatcata tgagaacatg      420

aactactacc ttaacttaat gattttgata aaaatcatta ggtacc                     466


<210>  71
<211>  494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNAI-R6K gamma origin 
       bacterial region. [NheI site-trpA terminator-R6K Origin-RNAI-KpnI
       site]

<400>  71
gctagcccgc ctaatgagcg ggcttttttt tggcttgttg tccacaaccg ttaaacctta       60

aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct      120

atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa      180

catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc      240

gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtacta tcaacaggtt      300

gaactgctga tccacgttgt gttaacttga agtggtggcc taactacggc tacactagaa      360

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta      420

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc      480

aggttaacgg tacc                                                        494


<210>  72
<211>  454
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R2 and NTC9385R2a 
       intronic R6K gamma origin- RSM bacterial region. Sequence show is
       O1; O2 is reverse complement

<400>  72
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtggttga catggtaaag agagttgtgt aaaatattga gtatgttcat      360

gttcttgtct ccttattgat ttttggggaa accatttgat catatgagaa catgaactac      420

taccttaact taatgatttt gataaaaatc atta                                  454


<210>  73
<211>  482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385R2 and NTC9385R2a 
       intronic R6K gamma origin- RNAI bacterial region. Sequence show 
       is O1; O2 is reverse complement

<400>  73
ccgcctaatg agcgggcttt tttttggctt gttgtccaca accgttaaac cttaaaagct       60

ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa      120

gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag      180

agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa      240

catgagagct tagtacgtta aacatgagag cttagtacgt actatcaaca ggttgaactg      300

ctgatccacg ttgtgttaac ttgaagtggt ggcctaacta cggctacact agaagaacag      360

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt      420

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcaggtta      480

ac                                                                     482


<210>  74
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RSM-ColE2 origin bacterial 
       region. [NheI site-ssiA-ColE2 Origin-RSM-KpnI site]

<400>  74
gctagctaca atggctcatg tggaaaaacc attggcagaa aaacacctgc caacagtttt       60

accacaattg ccacttaacc cacaaaaggg cgctgttatc tgataaggct tatctggtct      120

cattttgcac gttgtggttg acatggtaaa gagagttgtg taaaatattg agtatgttca      180

tgttcttgtc tccttattga tttttgggga aaccatttga tcatatgaga acatgaacta      240

ctaccttaac ttaatgattt tgataaaaat cattaggtac c                          281


<210>  75
<211>  309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: RNAI-ColE2 origin bacterial 
       region. [NheI site-ssiA-ColE2 Origin-RNAI-KpnI site]

<400>  75
gctagctaca atggctcatg tggaaaaacc attggcagaa aaacacctgc caacagtttt       60

accacaattg ccacttaacc cacaaaaggg cgctgttatc tgataaggct tatctggtct      120

cattttgcac gttgtgttaa cttgaagtgg tggcctaact acggctacac tagaagaaca      180

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct      240

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcaggtt      300

aacggtacc                                                              309


<210>  76
<211>  269
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C2 and NTC9385C2a 
       intronic C2 origin- RSM bacterial region. Sequence show is O1; O2
       is reverse complement

<400>  76
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg gttgacatgg taaagagagt tgtgtaaaat attgagtatg ttcatgttct      180

tgtctcctta ttgatttttg gggaaaccat ttgatcatat gagaacatga actactacct      240

taacttaatg attttgataa aaatcatta                                        269


<210>  77
<211>  297
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: NTC9385C2 and NTC9385C2a 
       intronic C2 origin- RNAI bacterial region. Sequence show is O1; 
       O2 is reverse complement

<400>  77
tacaatggct catgtggaaa aaccattggc agaaaaacac ctgccaacag ttttaccaca       60

attgccactt aacccacaaa agggcgctgt tatctgataa ggcttatctg gtctcatttt      120

gcacgttgtg ttaacttgaa gtggtggcct aactacggct acactagaag aacagtattt      180

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc      240

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca ggttaac         297


